Many of the Pinky and the Brain episodes occur in the 1990s at Acme Labs, located in some large American metropolitan city (presumably Burbank due to fact that the Warner Bros Animation Studio - which created this series and Animaniacs- is also located there) and underneath a suspension bridge. ' The B' rain: 'The same thing we do every night, Pinky-try to take over the world!' Pinky: 'Gee, Brain, what do you want to do tonight?' The opening song is preceded by the following dialogue: In common with many other Animaniacs shorts, many episodes are in some way a parody of something else, usually a film or novel. In each episode, Brain devises a new plan to take over the world, which ultimately ends in failure, usually due to Pinky's idiocy, the impossibility of Brain's plan, Brain's own arrogance, or just circumstances beyond their control.

Brain is self-centered and scheming Pinky is good-natured but feebleminded. Pinky and Brain are genetically enhanced laboratory mice who reside in a cage in the Acme Labs research facility. Later, they appeared in the unsuccessful series, Steven Spielberg Presents Pinky, Elmyra & the Brain.

From 1995 to 1998, Pinky and the Brain were spun off into their own show on The WB Television Network, Steven Spielberg Presents Pinky and the Brain, with 65 episodes produced by Steven Spielberg and Warner Bros. The characters Pinky and the Brain first appeared in 1993 as a recurring segment on the show Animaniacs. It was the first animated television series to be presented in Dolby Surround. Pinky and the Brain is an American animated television series.

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